Women of reproductive age are at the greatest risk of negative health effects from Zika virus, but current, nationally representative information on women’s knowledge about Zika is limited. This brief uses data from late-summer 2016 to explore women’s Zika awareness and concern, knowledge of Zika transmission modes, symptoms, and health effects, and actions taken to prevent Zika infection. The vast majority of women of reproductive age have knowledge gaps about Zika virus: more than half do not know that Zika can be sexually transmitted and less that one-third are aware that Zika can cause an infection with no symptoms.