Twenty-eight states and the federal government have enacted laws authorizing DNA collection from individuals arrested for or charged with certain offenses. Despite their widespread adoption, little is known about how these laws affect collecting agencies and crime laboratories responsible for their implementation. This article explores how key provisions in arrestee DNA legislation influence DNA collection and analysis. Information was derived from a review of state and federal laws and from interviews with crime laboratory representatives in 26 states that have passed arrestee DNA legislation. This data collection is part of an NIJ-funded Urban Institute project examining arrestee DNA collection.